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pME18S-F-R-hb2AR
(Plasmid #149367)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 149367 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    b2AR
  • Species
    H. sapiens (human)
  • Entrez Gene
    ADRB2 (a.k.a. ADRB2R, ADRBR, B2AR, BAR, BETA2AR)
  • Promoter SR alpha
  • Tags / Fusion Proteins
    • FLAG (N terminal on backbone)
    • rhodopsin (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site SacII (not destroyed)
  • 5′ sequencing primer GGCCTGTACGGAAGTGTTAC
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pME18S-F-R-hb2AR was a gift from Takeshi Imai (Addgene plasmid # 149367 ; http://n2t.net/addgene:149367 ; RRID:Addgene_149367)
  • For your References section:

    Widespread Inhibition, Antagonism, and Synergy in Mouse Olfactory Sensory Neurons In Vivo. Inagaki S, Iwata R, Iwamoto M, Imai T. Cell Rep. 2020 Jun 30;31(13):107814. doi: 10.1016/j.celrep.2020.107814. 10.1016/j.celrep.2020.107814 PubMed 32610120