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p15aTetT7mut-fuseYFP
(Plasmid #28013)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 28013 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pProlar.A122
  • Backbone manufacturer
    Clonetech
  • Backbone size w/o insert (bp) 2500
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5alpha pro, 37 degrees
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    EYFP-fuse, T7 RNAP
  • Species
    Synthetic, T7 phage
  • Insert Size (bp)
    3500

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site AvrII (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p15aTetT7mut-fuseYFP was a gift from Lingchong You (Addgene plasmid # 28013 ; http://n2t.net/addgene:28013 ; RRID:Addgene_28013)
  • For your References section:

    Emergent bistability by a growth-modulating positive feedback circuit. Tan C, Marguet P, You L. Nat Chem Biol. 2009 Nov . 5(11):842-8. 10.1038/nchembio.218 PubMed 19801994