-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 28012 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonepET15b
-
Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 6000
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Growth instructionsMC4100Z1 37 degrees C
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameT7 RNAP
-
SpeciesT7 phage
-
Insert Size (bp)2500
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site AatII (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pCFPT7 was a gift from Lingchong You (Addgene plasmid # 28012 ; http://n2t.net/addgene:28012 ; RRID:Addgene_28012) -
For your References section:
Emergent bistability by a growth-modulating positive feedback circuit. Tan C, Marguet P, You L. Nat Chem Biol. 2009 Nov . 5(11):842-8. 10.1038/nchembio.218 PubMed 19801994
Map uploaded by the depositor.
