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pCDNA-myc-HisA-Sirt3
(Plasmid #24924)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 24924 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA4/myc-His A
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5075
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Sirt3
  • Species
    H. sapiens (human)
  • Entrez Gene
    SIRT3 (a.k.a. SIR2L3)
  • Tags / Fusion Proteins
    • Myc (C terminal on insert)
    • His (C terminal on insert)

Cloning Information

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

NB: This construct was not used in this paper. The construct used in this paper is the HisB version.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA-myc-HisA-Sirt3 was a gift from Toren Finkel (Addgene plasmid # 24924 ; http://n2t.net/addgene:24924 ; RRID:Addgene_24924)
  • For your References section:

    A role for the mitochondrial deacetylase Sirt3 in regulating energy homeostasis. Ahn BH, Kim HS, Song S, Lee IH, Liu J, Vassilopoulos A, Deng CX, Finkel T. Proc Natl Acad Sci U S A. 2008 Sep 23. 105(38):14447-52. 10.1073/pnas.0803790105 PubMed 18794531