Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||24924||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepcDNA4/myc-His A
- Backbone size w/o insert (bp) 5075
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneSIRT3 (a.k.a. SIR2L3)
/ Fusion Proteins
- Myc (C terminal on insert)
- His (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
NB: This construct was not used in this paper. The construct used in this paper is the HisB version.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCDNA-myc-HisA-Sirt3 was a gift from Toren Finkel (Addgene plasmid # 24924 ; http://n2t.net/addgene:24924 ; RRID:Addgene_24924)
For your References section:A role for the mitochondrial deacetylase Sirt3 in regulating energy homeostasis. Ahn BH, Kim HS, Song S, Lee IH, Liu J, Vassilopoulos A, Deng CX, Finkel T. Proc Natl Acad Sci U S A. 2008 Sep 23. 105(38):14447-52. 10.1073/pnas.0803790105 PubMed 18794531