CS2 Flag-OAZ
(Plasmid
#14939)
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Depositing Lab
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Depositing OrganizationMemorial Sloan-Kettering Cancer Center
Located in United States of America -
Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 14939 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 14939-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 14939-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCS2
- Backbone size w/o insert (bp) 4100
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Vector typexenopus/mammalian/avian/zebrafish
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameOAZ
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SpeciesH. sapiens (human)
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Entrez GeneZNF423 (a.k.a. Ebfaz, JBTS19, NPHP14, OAZ, Roaz, ZFP423, Zfp104, hOAZ)
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Tag
/ Fusion Protein
- Flag (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer EBV rev
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
A human OAZ cDNA missing the first 18 codons (KIAA0760) was obtained from the Kazusa DNA Research Institute. A clone containing the amino-terminal end of human OAZ cDNA (ATCC Clone No. 2019478) was obtained from the EST database and sequenced. Full-length cDNA encoding human OAZ was constructed by fusing synthesized oligonucleotides encoding the first 18 amino acids to the KIAA0760 clone. A Flag epitope tag was introduced at the amino terminus of OAZ by PCR.
DNA (Catalog # 14939-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 14939-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CS2 Flag-OAZ was a gift from Joan Massague (Addgene plasmid # 14939 ; http://n2t.net/addgene:14939 ; RRID:Addgene_14939) -
For your References section:
OAZ uses distinct DNA- and protein-binding zinc fingers in separate BMP-Smad and Olf signaling pathways. Hata A, Seoane J, Lagna G, Montalvo E, Hemmati-Brivanlou A, Massague J. Cell. 2000 Jan 21. 100(2):229-40. 10.1016/S0092-8674(00)81561-5 PubMed 10660046
Map uploaded by the depositor.
