Pdx1 neo (DM#237)
(Plasmid #15084)

• Depositing Lab: Douglas Melton
• Publication: Rajagopal et al Science. 2003 Jan 17. 299(5605):363.

Backbone

• Vector backbone: na
• Vector type: Bacterial Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Pdx1 promoter
• Alt name: Pdx1-betageo
• Alt name: Pdx1
• Alt name: pIBHPT
• Species: M. musculus (mouse)
• Entrez Gene: Pdx1 (a.k.a. IDX-1, IPF-1, Ipf1, Mody4, STF-1, pdx-1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T3
• 3′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid is a knock in construct designed to sort out Pdx1 expressing cells by neomycin resistancy. See author's map for more information.