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optGluCl alpha EYFP
(Plasmid #15104)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 15104 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GluCl alpha
  • Alt name
    Glutamate-gated chloride channel alpha
  • Alt name
    GluCl
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    2100
  • Mutation
    Codon optimized cDNA
  • Tag / Fusion Protein
    • EYFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The lab has created an improved version of this plasmid, which can be found at http://www.addgene.org/47387/ .

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    optGluCl alpha EYFP was a gift from Henry Lester (Addgene plasmid # 15104 ; http://n2t.net/addgene:15104 ; RRID:Addgene_15104)
  • For your References section:

    Codon optimization of Caenorhabditis elegans GluCl ion channel genes for mammalian cells dramatically improves expression levels. Slimko EM, Lester HA. J Neurosci Methods. 2003 Mar 30. 124(1):75-81. 10.1016/S0165-0270(02)00362-X PubMed 12648766