Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||15126||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size (bp) 5400
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
/ Fusion Protein
- HA (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
pC.HA was derived from plasmid pCG (Tanaka, M, and Herr, W., 1990. Cell 60:375-386). pCG contains the human CMV promoter, the HSV tk gene 5' untranslated leader and initiation codon, rabbit beta-globin gene splicing and polyA singals, and the replicon origin of SV40.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pC.HA was a gift from Joan Brugge (Addgene plasmid # 15126 ; http://n2t.net/addgene:15126 ; RRID:Addgene_15126)
For your References section:Vav family proteins couple to diverse cell surface receptors. Moores SL, Selfors LM, Fredericks J, Breit T, Fujikawa K, Alt FW, Brugge JS, Swat W. Mol Cell Biol. 2000 Sep . 20(17):6364-73. 10.1128/MCB.20.17.6364-6373.2000 PubMed 10938113