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Flag-ECFP-Paxillin
(Plasmid #15211)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 15211 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 6100
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    paxillin
  • Species
    G. gallus (chicken)
  • Insert Size (bp)
    1700
  • GenBank ID
    L30099
  • Entrez Gene
    PXN (a.k.a. TGFB1I1)
  • Tags / Fusion Proteins
    • Flag (N terminal on backbone)
    • ECFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (not destroyed)
  • 3′ cloning site EcoR1 (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer Sp6
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Chris Turner SUNY Upstate Medical University

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Flag-ECFP-Paxillin was a gift from Rick Horwitz (Addgene plasmid # 15211 ; http://n2t.net/addgene:15211 ; RRID:Addgene_15211)
  • For your References section:

    Paxillin phosphorylation sites mapped by mass spectrometry. Webb DJ, Schroeder MJ, Brame CJ, Whitmore L, Shabanowitz J, Hunt DF, Horwitz AR. J Cell Sci. 2005 Nov 1. 118(Pt 21):4925-9. 10.1242/jcs.02563 PubMed 16254239