GIT1A-FLAG
(Plasmid #15225)

• Depositing Lab: Rick Horwitz
• Publication: Manabe et al J Cell Sci. 2002 Apr 1. 115(Pt 7):1497-510.

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	15225	Standard format: Plasmid sent in bacteria as agar stab	1	$65	Add to Cart

This material is available to academics and nonprofits only.

Backbone

• Vector backbone: pEGFP-N1
  (Search Vector Database)
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4000
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: G protein coupled receptor kinase-interacting protein 1
• Alt name: GIT1
• Species: H. sapiens (human)
• Insert Size (bp): 2200
• Entrez Gene: GIT1
• Tag / Fusion Protein:
  • flag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Xho1 (destroyed during cloning)
• 3′ cloning site: Not1 (not destroyed)
• 5′ sequencing primer: CMV immediate early gene forward primer

Resource Information

• Terms and Licenses:
  • UBMTA
  • Ancillary Agreement for Plasmids Containing FP Materials
• Articles Citing this Plasmid:
  • 3 References