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GIT1A-FLAG
(Plasmid #15225)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 15225 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4000
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    G protein coupled receptor kinase-interacting protein 1
  • Alt name
    GIT1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2200
  • Entrez Gene
    GIT1 (a.k.a. p95-APP1)
  • Tag / Fusion Protein
    • flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xho1 (destroyed during cloning)
  • 3′ cloning site Not1 (not destroyed)
  • 5′ sequencing primer CMV immediate early gene forward primer
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene QC sequence analysis identified L425V, E476K, and L583P discrepancies relative to Genbank ID: NP_001078923.1. It is unknown what, if any, impact these may have on plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GIT1A-FLAG was a gift from Rick Horwitz (Addgene plasmid # 15225 ; http://n2t.net/addgene:15225 ; RRID:Addgene_15225)
  • For your References section:

    GIT1 functions in a motile, multi-molecular signaling complex that regulates protrusive activity and cell migration. Manabe R, Kovalenko M, Webb DJ, Horwitz AR. J Cell Sci. 2002 Apr 1. 115(Pt 7):1497-510. PubMed 11896197