GIT1A-FLAG
(Plasmid #15225)

• Depositing Lab: Rick Horwitz
• Publication: Manabe et al J Cell Sci. 2002 Apr 1. 115(Pt 7):1497-510.

Backbone

• Vector backbone: pEGFP-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4000
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: G protein coupled receptor kinase-interacting protein 1
• Alt name: GIT1
• Species: H. sapiens (human)
• Insert Size (bp): 2200
• Entrez Gene: GIT1 (a.k.a. p95-APP1)
• Tag / Fusion Protein:
  • flag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Xho1 (destroyed during cloning)
• 3′ cloning site: Not1 (not destroyed)
• 5′ sequencing primer: CMV immediate early gene forward primer

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene QC sequence analysis identified L425V, E476K, and L583P discrepancies relative to Genbank ID: NP_001078923.1. It is unknown what, if any, impact these may have on plasmid function.