OA-1136J
(Plasmid
#153023)
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PurposeExpress His-MBP-TEV-CasRx recombinant protein
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 153023 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepET His6 MBP TEV LIC cloning vector (1M)
- Backbone size w/o insert (bp) 6430
- Total vector size (bp) 9328
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCasRx
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Alt nameNLS-RfxCas13d-NLS
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SpeciesRuminococcus flavefaciens
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Insert Size (bp)2898
- Promoter T7
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Tag
/ Fusion Protein
- His-MBP-TEV (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer unknown
- 3′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
OA-1136J was a gift from Omar Akbari (Addgene plasmid # 153023 ; http://n2t.net/addgene:153023 ; RRID:Addgene_153023) -
For your References section:
Development of a Rapid and Sensitive CasRx-Based Diagnostic Assay for SARS-CoV-2. Brogan DJ, Chaverra-Rodriguez D, Lin CP, Smidler AL, Yang T, Alcantara LM, Antoshechkin I, Liu J, Raban RR, Belda-Ferre P, Knight R, Komives EA, Akbari OS. ACS Sens. 2021 Nov 26;6(11):3957-3966. doi: 10.1021/acssensors.1c01088. Epub 2021 Oct 29. 10.1021/acssensors.1c01088 PubMed 34714054
