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pCD442
(Plasmid #153024)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 153024 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dCas9, MCP-SoxS(R93A/S101A)
  • Species
    S. pyogenes (dCas9), MS2 (MCP), E. coli (SoxS)
  • Mutation
    SoxS has R93A and S101A mutations

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCD442 was a gift from Jesse Zalatan (Addgene plasmid # 153024 ; http://n2t.net/addgene:153024 ; RRID:Addgene_153024)
  • For your References section:

    Effective CRISPRa-mediated control of gene expression in bacteria must overcome strict target site requirements. Fontana J, Dong C, Kiattisewee C, Chavali VP, Tickman BI, Carothers JM, Zalatan JG. Nat Commun. 2020 Apr 1;11(1):1618. doi: 10.1038/s41467-020-15454-y. 10.1038/s41467-020-15454-y PubMed 32238808