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Effective CRISPRa-mediated control of gene expression in bacteria must overcome strict target site requirements.
Fontana J, Dong C, Kiattisewee C, Chavali VP, Tickman BI, Carothers JM, Zalatan JG
Nat Commun. 2020 Apr 1;11(1):1618. doi: 10.1038/s41467-020-15454-y.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
153024pCD442dCas9, MCP-SoxS(R93A/S101A)
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153025pCK005.6dCas9, MCP-SoxS(R93A/S101A), J106 scRNA
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153026pCD564dxCas9(3.7), MCP-SoxS(R93A/S101A)
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153027pCD565dxCas9(3.7), MCP-SoxS(R93A/S101A), J306 scRNA
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153028pJF143.J3J3-mRFP1
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