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(Plasmid #153027)


Item Catalog # Description Quantity Price (USD)
Plasmid 153027 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    dxCas9(3.7), MCP-SoxS(R93A/S101A), J306 scRNA
  • Species
    Evolved (dxCas9(3.7)), MS2 (MCP), E. coli (SoxS), synthetic (J306 scRNA)
  • Mutation
    SoxS has R93A and S101A mutations

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    dxCas9(3.7) was a gift from David Liu
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCD565 was a gift from Jesse Zalatan (Addgene plasmid # 153027 ; ; RRID:Addgene_153027)
  • For your References section:

    Effective CRISPRa-mediated control of gene expression in bacteria must overcome strict target site requirements. Fontana J, Dong C, Kiattisewee C, Chavali VP, Tickman BI, Carothers JM, Zalatan JG. Nat Commun. 2020 Apr 1;11(1):1618. doi: 10.1038/s41467-020-15454-y. 10.1038/s41467-020-15454-y PubMed 32238808