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(Plasmid #153085)


Item Catalog # Description Quantity Price (USD)
Plasmid 153085 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
    pAAV CW3SL-eGFP (Addgene 61463)
  • Backbone size w/o insert (bp) 3860
  • Total vector size (bp) 6650
  • Modifications to backbone
    CamKII promoter was replaced by synapsin promoter. EGFP removed.
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Researchers should avoid repetitive freeze-thaw cycles when handling the DNA, as we found that this can lead to DNA degradation. Recommended growth strains are XL10gold.
  • Copy number

Gene/Insert 1

  • Gene/Insert name
  • Alt name
    photoactivated adenylyl cyclase from Beggiatoa sp.
  • Species
    Beggiatoa sp.
  • Insert Size (bp)
  • GenBank ID
  • Tags / Fusion Proteins
    • myc tag (C terminal on insert)
    • His tag (C terminal on insert)

Gene/Insert 2

  • Gene/Insert name
  • Alt name
  • Species
    R. norvegicus (rat)
  • GenBank ID
  • Entrez Gene
    Syp (a.k.a. Syp1)
  • Tag / Fusion Protein
    • mScarlet (C terminal on insert)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-syn-synaptoPAC-minWPRE was a gift from Dietmar Schmitz (Addgene plasmid # 153085 ; ; RRID:Addgene_153085)