pET30a_HIV-RT
(Plasmid #153311)

• Purpose: Bacterial expression of HIV reverse transcriptase
• Depositing Labs: Bryan Dickinson, Drew Endy
• Publication: COVID-19 Diagnostic Toolkit Enzymes (unpublished)

Backbone

• Vector backbone: pET30a
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HIV Reverse Transcriptase
• Species: HIV
• Insert Size (bp): 1716
• Tag / Fusion Protein:
  • His (N terminal on backbone)

Cloning Information

• Cloning method: Unknown

Resource Information

• Supplemental Documents:
  • pET30a_HIV-RT.gbk
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • OpenMTA
• Industry Terms:
  • OpenMTA

Depositor Comments

Plasmid made by Bryan Dickinson and Huiqing Zhou at University of Chicago