pRepair-mScI-CTNNB1
(Plasmid
#153431)
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PurposeHomology Directed Repair construct for N-terminal tagging of hsCTNNB1 with mScarlet-i
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Depositing Lab
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Depositing OrganizationUniversity of Amsterdam
Located in the Netherlands -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 153431 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 153431-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 153431-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepBluescript KS (+)
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Backbone manufacturerstratagene
- Backbone size w/o insert (bp) 2965
- Total vector size (bp) 5261
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Vector typeCRISPR ; HDR donor template
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCTNNB1 homology arms and mScI coding sequence
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Alt nameCTNNB1
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SpeciesH. sapiens (human)
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Insert Size (bp)2296
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GenBank ID
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Entrez GeneCTNNB1 (a.k.a. CTNNB, EVR7, MRD19, NEDSDV, armadillo)
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Tag
/ Fusion Protein
- mScarlet-i (N terminal on insert)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
mScI was cloned from pmScarlet-i_C1 (Dorus Gadella, Plasmid #85044)
Homology arms were cloned from genomic HEK293 DNA. Please visit https://www.biorxiv.org/content/10.1101/2020.05.28.120543v1 for bioRxiv preprint.
DNA (Catalog # 153431-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 153431-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRepair-mScI-CTNNB1 was a gift from Renee van Amerongen (Addgene plasmid # 153431 ; http://n2t.net/addgene:153431 ; RRID:Addgene_153431) -
For your References section:
Quantitative live-cell imaging and computational modeling shed new light on endogenous WNT/CTNNB1 signaling dynamics. de Man SM, Zwanenburg G, van der Wal T, Hink MA, van Amerongen R. Elife. 2021 Jun 30;10. pii: 66440. doi: 10.7554/eLife.66440. 10.7554/eLife.66440 PubMed 34190040
Map uploaded by the depositor.
