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(Plasmid #153430)


Item Catalog # Description Quantity Price (USD)
Plasmid 153430 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    pBluescript KS (+)
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 2965
  • Total vector size (bp) 5282
  • Vector type
    CRISPR ; HDR donor template

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    CTNNB1 homology arms and mTurquoise2 coding sequence
  • Alt name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • GenBank ID
  • Entrez Gene
    CTNNB1 (a.k.a. CTNNB, EVR7, MRD19, NEDSDV, armadillo)
  • Tag / Fusion Protein
    • SGFP2 (N terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

SGFP2 was cloned from pSGFP2-C1 (Dorus Gadella, Plasmid #22881)
Homology arms were cloned from genomic HEK293 DNA. Please visit for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRepair-SGFP2-CTNNB1 was a gift from Renee van Amerongen (Addgene plasmid # 153430 ; ; RRID:Addgene_153430)
  • For your References section:

    Quantitative live-cell imaging and computational modeling shed new light on endogenous WNT/CTNNB1 signaling dynamics. de Man SM, Zwanenburg G, van der Wal T, Hink MA, van Amerongen R. Elife. 2021 Jun 30;10. pii: 66440. doi: 10.7554/eLife.66440. 10.7554/eLife.66440 PubMed 34190040