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Addgene

pcDNA3.3-3XFLAG-AGO1
(Plasmid #153556)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 153556 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pcDNA3.3
  • Backbone size w/o insert (bp) 5494
  • Total vector size (bp) 8065
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    argonaute RISC Catalytic Component 1
  • Alt name
    AGO1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2571
  • Entrez Gene
    AGO1 (a.k.a. EIF2C, EIF2C1, GERP95, NEDLBAS, Q99, hAgo1)
  • Promoter CMV
  • Tag / Fusion Protein
    • 3XFLAG (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.3-3XFLAG-AGO1 was a gift from David Bartel (Addgene plasmid # 153556 ; http://n2t.net/addgene:153556 ; RRID:Addgene_153556)

  • For your References section:

    The biochemical basis for the cooperative action of microRNAs. Briskin D, Wang PY, Bartel DP. Proc Natl Acad Sci U S A. 2020 Jul 28;117(30):17764-17774. doi: 10.1073/pnas.1920404117. Epub 2020 Jul 13. 10.1073/pnas.1920404117 PubMed 32661162
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