pGBW-m4252802
(Plasmid
#153679)
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PurposeMammalian expression plasmid for SARS-CoV-2 nsp11
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Depositing Labs
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Depositing OrganizationGinkgo Bioworks
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 153679 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 * | |
| DNA | 153679-D50 | 50 μg of DNA in Tris buffer | $495 * | ||
| DNA | 153679-D100 | 100 μg of DNA in Tris buffer | $585 * | ||
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Backbone
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Vector backbonepTwist_CMV_Puro_6 with AarI site removed
- Backbone size w/o insert (bp) 6080
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSARS-CoV-2 nsp11
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SpeciesSevere acute respiratory syndrome coronavirus 2
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Insert Size (bp)32
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Mutationwt
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Entrez GeneORF1ab (a.k.a. GU280_gp01)
- Promoter CMV
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Tag
/ Fusion Protein
- Cleavable TEV;3xFlag (C terminal on backbone)
Cloning Information
- Cloning method Unknown
Resource Information
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A portion of this plasmid was derived from a plasmid made byGinkgoBioworks
Terms and Licenses
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
NCBI reference sequence: 43740578
DNA (Catalog # 153679-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 153679-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGBW-m4252802 was a gift from Ginkgo Bioworks & Benjie Chen (Addgene plasmid # 153679 ; http://n2t.net/addgene:153679 ; RRID:Addgene_153679)
