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(Plasmid #153859)


Item Catalog # Description Quantity Price (USD)
Plasmid 153859 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *

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  • Vector backbone
    ori-013 - marker | cmR
  • Backbone size w/o insert (bp) 1462
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    fragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTR
  • Species
    Severe acute respiratory syndrome coronavirus 2
  • Insert Size (bp)
  • Mutation
    Nearly native encoding;handful SNPs as watermarks; tIIs restriction sites removed with 15 base polyA tail
  • Entrez Gene
    ORF1ab (a.k.a. GU280_gp01)
  • Promoter Promoter | t7 consensus from NEB, G is +1

Cloning Information

  • Cloning method Unknown

Resource Information

  • A portion of this plasmid was derived from a plasmid made by

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid contains a SARS-CoV-2 viral sequence fragment that covers basepairs 8555 to the end of the genome. Because of sequence complexities in nsp3, it is difficult to synthesize the complete nsp3 gene. This construct may be useful to produce RNA controls for assays, as well as serve as templates to make antigens.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGBW-m4092709 was a gift from Ginkgo Bioworks & Benjie Chen (Addgene plasmid # 153859 ; ; RRID:Addgene_153859)