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(Plasmid #153907)


Item Catalog # Description Quantity Price (USD)
Plasmid 153907 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *

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  • Vector backbone
    pG9m-2 backbone
  • Backbone size w/o insert (bp) 1968
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy


  • Gene/Insert name
    fragment SARS-CoV-2 orf1a mature peptide 3 to orf1a stop
  • Species
    Severe acute respiratory syndrome coronavirus 2
  • Insert Size (bp)
  • Mutation
  • Entrez Gene
    ORF1ab (a.k.a. GU280_gp01)
  • Promoter Promoter | t7 consensus from NEB, G is +1

Cloning Information

  • Cloning method Unknown

Resource Information

  • A portion of this plasmid was derived from a plasmid made by

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid contains a SARS-CoV-2 viral sequence fragment that covers basepairs 8555 to the end of the genome. Because of sequence complexities in nsp3, it is difficult to synthesize the complete nsp3 gene. This construct may be useful to produce RNA controls for assays, as well as serve as templates to make antigens.

During Addgene quality control, an IS3 element and transposase were identified downstream from the SARS-CoV-2 fragment. Requesting scientists should validate that the element has not further transposed into essential elements prior to experimental use.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGBW-m4092694 was a gift from Ginkgo Bioworks & Benjie Chen (Addgene plasmid # 153907 ; ; RRID:Addgene_153907)