PurposeBacterial expression plasmid for fragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTR
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||153895||Standard format: Plasmid sent in bacteria as agar stab||1||$75 *|
* Login to view industry pricing.
Vector backbonepG9m-2 backbone
- Backbone size w/o insert (bp) 1968
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberLow Copy
Gene/Insert namefragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTR
SpeciesSevere acute respiratory syndrome coronavirus 2
Insert Size (bp)8319
MutationNearly native encoding;inactivating mutations in all ORFs and tIIs restriction sites removed with 5 base polyA tail
Entrez GeneORF1ab (a.k.a. GU280_gp01)
- Promoter Promoter | t7 consensus from NEB, G is +1
- Cloning method Unknown
A portion of this plasmid was derived from a plasmid made byGinkgoBioworks
Terms and Licenses
- Zeocin® is an InvivoGen trademark.
This plasmid contains a SARS-CoV-2 viral sequence fragment that covers basepairs 8555 to the end of the genome. Because of sequence complexities in nsp3, it is difficult to synthesize the complete nsp3 gene. This construct may be useful to produce RNA controls for assays, as well as serve as templates to make antigens.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGBW-m4092686 was a gift from Ginkgo Bioworks & Benjie Chen (Addgene plasmid # 153895 ; http://n2t.net/addgene:153895 ; RRID:Addgene_153895)