pMKO.1 puro-luc (shRNA)
(Plasmid #153962)

• Purpose: Expresses a shRNA against firefly luciferase
• Depositing Lab: Hiroyuki Konishi
• Publication: Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7. doi: 10.1016/j.celrep.2019.12.064.

Backbone

• Vector backbone: pMKO.1 puro (Addgene #8452)
• Backbone manufacturer: Robert Weinberg
• Backbone size w/o insert (bp): 6340
• Total vector size (bp): 6371
• Modifications to backbone: This plasmid was made by removing a shRNA against p53 from pMKO.1 puro p53 shRNA 2 (Addgene #10672, provided by William Hahn) and instead incorporating a shRNA against firefly luciferase (shVC)
• Vector type: Mammalian Expression, Retroviral, RNAi, Luciferase
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: shRNA against firefly luciferase (19-bp-long target)
• Alt name: shVC
• gRNA/shRNA sequence: CTTACGCTGAGTACTTCGA
• Species: Synthetic; from firefly
• Promoter: U6 promoter
• Tag / Fusion Protein:
  • N/A

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: -
• 3′ sequencing primer: -

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry