pNM3746
(Plasmid #154109)

• Purpose: loxP FLP FRT FRT3 landing site in a Golden Gate SapI GCG ACG assembly vector
• Depositing Lab: Michael Nonet
• Publication: Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.303388. doi: 10.1534/genetics.120.303388.

Backbone

• Vector backbone: DR274
• Backbone manufacturer: Addgene #42250
• Backbone size w/o insert (bp): 1857
• Total vector size (bp): 13452
• Modifications to backbone: Kan Resistant HindIII-SwaI 1859 bp fragment of DR274 lacking T7 promoter and sgRNA scaffold used as backbone.
• Vector type: Worm Expression, Cre/Lox ; RMCE
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: LoxP-SEC-LoxP
• Species: C. elegans (nematode), S. cerevisiae (budding yeast), Synthetic
• Insert Size (bp): 5617
• Promoter: not applicable

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: not applicable

Gene/Insert 2

• Gene/Insert name: FRT::rpl-28p::GFP::His-58::FRT3
• Species: C. elegans (nematode), S. cerevisiae (budding yeast), Synthetic
• Promoter: not applicable

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: not applicable

Gene/Insert 3

• Gene/Insert name: mex-5p::FLP D5::sl2::mNG::glh2 3'
• Species: C. elegans (nematode), S. cerevisiae (budding yeast), Synthetic
• Insert Size (bp): 3565
• Promoter: not applicable

Cloning Information for Gene/Insert 3

• Cloning method: Unknown
• 5′ sequencing primer: not applicable

Resource Information

• Supplemental Documents:
  • pNM3746.gb
• A portion of this plasmid was derived from a plasmid made by: LoxP-SEC-LoxP from addgene #91829, mex-5p::FLP D5::sl2::mNG::glh-2 from Addgene #109326, rpl-28p from genomic DNA, FRT::GFP::his-58 from Addgene #86718 , and FRT3 synthetic. See Nonet (2020) Genetics for precise assembly description.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • mNeonGreen Label License
• Industry Terms:
  • Not Available to Industry