pNM3689
(Plasmid #154111)

• Purpose: miniMos single component RMCE landing site vector
• Depositing Lab: Michael Nonet
• Publication: Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.303388. doi: 10.1534/genetics.120.303388.

Backbone

• Vector backbone: pCFJ1272
• Backbone manufacturer: Addgene #44486
• Backbone size w/o insert (bp): 3173
• Total vector size (bp): 14826
• Vector type: miniMos
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: loxP::SEC::loxP::mex-5p::FLP::sl2::nNeonGreen::glh-2 3'::rpl-28p::FRT::GFP::his-58::unc-54 3'::FRT3
• Species: C. elegans (nematode), S. cerevisiae (budding yeast), Synthetic
• Insert Size (bp): 11680
• Promoter: not applicable

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: not applicable

Resource Information

• Supplemental Documents:
  • pNM3689.gb
• A portion of this plasmid was derived from a plasmid made by: The insert was assembled using a multi-fragment Golden Gate reaction amplifying Addgene #154110 in two fragments, and inserting the mex-5p:FLP::sl2::mNeonGreen::glh-2 3' sequences from C. elegans genomic DNA of animals carrying addgene plasmid #109326. See Nonet (2020) Genetics for precise details.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • mNeonGreen Label License
• Industry Terms:
  • Not Available to Industry