pGEMHE-TNKS-2
(Plasmid #154149)

• Purpose: For making TNKS-2 RNA
• Depositing Lab: Carmen Williams
• Publication: Gambini et al Dev Cell. 2020 Jun 8;53(5):545-560.e7. doi: 10.1016/j.devcel.2020.04.018. Epub 2020 May 21.

Backbone

• Vector backbone: pGEMHE
• Backbone manufacturer: Addgene
• Backbone size w/o insert (bp): 3064
• Total vector size (bp): 6601
• Vector type: in vitro transcription

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Tankyrase 2
• Alt name: TANKS2
• Species: H. sapiens (human)
• Insert Size (bp): 3537
• GenBank ID: NM_025235.4
• Entrez Gene: TNKS2 (a.k.a. ARTD6, PARP-5b, PARP-5c, PARP5B, PARP5C, TANK2, TNKL, pART6)
• Promoter: T7
• Tag / Fusion Protein:
  • FLAG (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Sal1 (not destroyed)
• 3′ cloning site: Xba1 (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: GGCTTCCTCTAGATTATCCATCGACCATACCTTC

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Addgene Cat##34691

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry