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pLL29
(Plasmid #15457)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 15457 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    N/A
  • Backbone size w/o insert (bp) 7029
  • Vector type
    S. aureus-E. coli shuttle vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    tetracycline/ S. aureus
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    None
  • Insert Size (bp)
    7029
  • GenBank ID
    EF540895

Cloning Information

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

pCL25 can integrate into the L54a attB site, whereas pLL29 and pLL39 can integrate at either one of the two sites: L54a attB and phi-11 attB.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLL29 was a gift from Chia Lee (Addgene plasmid # 15457 ; http://n2t.net/addgene:15457 ; RRID:Addgene_15457)
  • For your References section:

    Improved single-copy integration vectors for Staphylococcus aureus. Luong TT, Lee CY. J Microbiol Methods. 2007 Jul . 70(1):186-90. 10.1016/j.mimet.2007.04.007 PubMed 17512993