pMS4 (Empty insertion vector with SEC)
(Plasmid #154837)

• Purpose: Vector that can be modified to add gene(s) of interest to C. elegans ChrII:8420157 site via CRISPR with SEC selection
• Depositing Lab: Patrick Phillips
• Publication: Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.401400. doi: 10.1534/g3.120.401400.

Backbone

• Vector backbone: pCFJ151
• Backbone size w/o insert (bp): 5049
• Total vector size (bp): 10695
• Modifications to backbone: Deletion of Cbr-unc-119 rescue gene and a portion of the homology arm containing the targeted guide site.
• Vector type: Worm Expression, Cre/Lox, CRISPR
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SEC
• Alt name: sqt-1(d)::hsCre::hygR
• Species: C. elegans (nematode), Synthetic
• Insert Size (bp): 5623
• Mutation: sqt-1(e1350)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: gcccaggagaacacgttag
• 3′ sequencing primer: Gcttatctcgaatgagacccttg

Resource Information

• Supplemental Documents:
  • pMS4.gb
• A portion of this plasmid was derived from a plasmid made by: The vector backbone (pCFJ151) and the SEC insert (from pDD285) were received from Addgene

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Additional protocol information can be found at: github.com/phillips-lab/SLP