Lenti-SLC35B2-sgRNA
(Plasmid #154860)

• Purpose: Lentiviral expression of Cas9 and sgRNA targeting SLC35B2
• Depositing Lab: Yihong Ye
• Publication: Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;117(20):10865-10875. doi: 10.1073/pnas.1918617117. Epub 2020 May 4.

Backbone

• Vector backbone: lentiCRISPR v2 (addgene# 52961)
• Vector type: Mammalian Expression, Lentiviral, CRISPR
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SLC35B2 sgRNA
• Alt name: PAPST1 sgRNA
• gRNA/shRNA sequence: TCCGCCTGAAGTACTGCACC
• Species: H. sapiens (human)
• GenBank ID: NM_178148
• Entrez Gene: SLC35B2 (a.k.a. HLD26, PAPST1, SLL, UGTrel4)
• Promoter: U6

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsmBI (destroyed during cloning)
• 3′ cloning site: BsmBI (destroyed during cloning)
• 5′ sequencing primer: GAGGGCCTATTTCCCATGATT
• 3′ sequencing primer: CACAGAGTTGGTGCCGATGGCCAGGCCGATGCTGTACTT

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry