Skip to main content
Addgene
Open Menu

pPB-tetpA(hBCL2-miR9/9*-124)-iRPT
(Plasmid #154881)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 154881 Standard format: Plasmid sent in bacteria as agar stab 1 $94
Add to Cart
DNA 154881-D50 50 μg of DNA in Tris buffer $495
Add to Cart
DNA 154881-D100 100 μg of DNA in Tris buffer $585
Add to Cart

Backbone

  • Vector backbone
    pPB-tetpA (Addgene #140763)
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    iRFP713
  • Species
    Synthetic
  • Insert Size (bp)
    948

Gene/Insert 2

  • Gene/Insert name
    Puromycin resistance gene
  • Species
    E.coli
  • Insert Size (bp)
    597

Gene/Insert 3

  • Gene/Insert name
    rtTAM2
  • Species
    Synthetic
  • Insert Size (bp)
    747

Gene/Insert 4

  • Gene/Insert name
    BCL2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    717
  • Entrez Gene
    BCL2 (a.k.a. Bcl-2, PPP1R50)

Gene/Insert 5

  • Gene/Insert name
    miR9/9*-124
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    656

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPB-tetpA(hBCL2-miR9/9*-124)-iRPT was a gift from Hideyuki Okano (Addgene plasmid # 154881 ; http://n2t.net/addgene:154881 ; RRID:Addgene_154881)

  • For your References section:

    Direct Neuronal Reprogramming of Common Marmoset Fibroblasts by ASCL1, microRNA-9/9*, and microRNA-124 Overexpression. Nemoto A, Kobayashi R, Yoshimatsu S, Sato Y, Kondo T, Yoo AS, Shiozawa S, Okano H. Cells. 2020 Dec 22;10(1). pii: cells10010006. doi: 10.3390/cells10010006. 10.3390/cells10010006 PubMed 33375083
Related items:
Commonly requested with: