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PurposeEpisomal expression of reprogramming factors.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 165081 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMaster
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Vector typeMammalian Expression ; Episomal
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Selectable markersNeomycin (select with G418), Puromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Growth instructionsPlasmid is unstable and prone to recombination. Test 3-5 small colonies to ensure the full plasmid is intact.
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameOCT4
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Alt namePOU5F1
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SpeciesH. sapiens (human)
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Insert Size (bp)1080
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Entrez GenePOU5F1 (a.k.a. OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4)
Gene/Insert 2
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Gene/Insert nameSOX2
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SpeciesH. sapiens (human)
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Insert Size (bp)951
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Entrez GeneSOX2 (a.k.a. ANOP3, MCOPS3)
Gene/Insert 3
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Gene/Insert nameKLF4
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SpeciesH. sapiens (human)
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Insert Size (bp)1410
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Entrez GeneKLF4 (a.k.a. EZF, GKLF)
Gene/Insert 4
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Gene/Insert nameMYC
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Alt nameC-MYC
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SpeciesH. sapiens (human)
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Insert Size (bp)1320
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Entrez GeneMYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)
Gene/Insert 5
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Gene/Insert nameLRH1
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SpeciesH. sapiens (human)
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Insert Size (bp)1488
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Entrez GeneNR5A2 (a.k.a. B1F, B1F2, CPF, FTF, FTZ-F1, FTZ-F1beta, LRH-1, LRH1, hB1F-2)
Gene/Insert 6
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Gene/Insert nameNANOG
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SpeciesH. sapiens (human)
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Insert Size (bp)915
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Entrez GeneNANOG
Gene/Insert 7
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Gene/Insert namelin28
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SpeciesH. sapiens (human)
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Insert Size (bp)627
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Entrez GeneLIN28A (a.k.a. CSDD1, LIN-28, LIN28, ZCCHC1, lin-28A)
Gene/Insert 8
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Gene/Insert nameEGFP
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SpeciesAequorea victoria
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Insert Size (bp)710
Gene/Insert 9
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Gene/Insert nameHSV TK
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SpeciesHerpes Simplex Virus
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Insert Size (bp)1131
Gene/Insert 10
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Gene/Insert nameKDM4D
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SpeciesCallithrix jacchus
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Insert Size (bp)1569
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Entrez GeneKDM4D (a.k.a. JMJD2D)
Gene/Insert 11
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Gene/Insert nameGLIS1
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SpeciesH. sapiens (human)
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Insert Size (bp)1860
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Entrez GeneGLIS1
Resource Information
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Supplemental Documents
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Addgene Notes
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that this plasmid contains repetitive elements which are prone to recombination. Screen 3-5 small colonies to ensure the full plasmid is selected. For example, the restriction enzyme NheI can be used to check the plasmid size as shown in Addgene's Diagnostic Digest.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMasterK was a gift from Hideyuki Okano (Addgene plasmid # 165081) -
For your References section:
Establishing an induced pluripotent stem cell line from neonatal common marmoset fibroblasts by an all-in-one episomal vector approach. Yoshimatsu S, Qian E, Sato T, Yamamoto M, Ishikawa M, Okano H. Stem Cell Res. 2021 May;53:102380. doi: 10.1016/j.scr.2021.102380. Epub 2021 Apr 30. 10.1016/j.scr.2021.102380 PubMed 34088009