pMasterK
(Plasmid #165081)

• Purpose: Episomal expression of reprogramming factors.
• Depositing Lab: Hideyuki Okano
• Publication: Yoshimatsu et al Stem Cell Res. 2021 May;53:102380. doi: 10.1016/j.scr.2021.102380. Epub 2021 Apr 30.

Backbone

• Vector backbone: pMaster
• Vector type: Mammalian Expression ; Episomal
• Selectable markers: Neomycin (select with G418), Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Growth instructions: Plasmid is unstable and prone to recombination. Test 3-5 small colonies to ensure the full plasmid is intact.
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: OCT4
• Alt name: POU5F1
• Species: H. sapiens (human)
• Insert Size (bp): 1080
• Entrez Gene: POU5F1 (a.k.a. OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4)

Gene/Insert 2

• Gene/Insert name: SOX2
• Species: H. sapiens (human)
• Insert Size (bp): 951
• Entrez Gene: SOX2 (a.k.a. ANOP3, MCOPS3)

Gene/Insert 3

• Gene/Insert name: KLF4
• Species: H. sapiens (human)
• Insert Size (bp): 1410
• Entrez Gene: KLF4 (a.k.a. EZF, GKLF)

Gene/Insert 4

• Gene/Insert name: MYC
• Alt name: C-MYC
• Species: H. sapiens (human)
• Insert Size (bp): 1320
• Entrez Gene: MYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)

Gene/Insert 5

• Gene/Insert name: LRH1
• Species: H. sapiens (human)
• Insert Size (bp): 1488
• Entrez Gene: NR5A2 (a.k.a. B1F, B1F2, CPF, FTF, FTZ-F1, FTZ-F1beta, LRH-1, LRH1, hB1F-2)

Gene/Insert 6

• Gene/Insert name: NANOG
• Species: H. sapiens (human)
• Insert Size (bp): 915
• Entrez Gene: NANOG

Gene/Insert 7

• Gene/Insert name: lin28
• Species: H. sapiens (human)
• Insert Size (bp): 627
• Entrez Gene: LIN28A (a.k.a. CSDD1, LIN-28, LIN28, ZCCHC1, lin-28A)

Gene/Insert 8

• Gene/Insert name: EGFP
• Species: Aequorea victoria
• Insert Size (bp): 710

Gene/Insert 9

• Gene/Insert name: HSV TK
• Species: Herpes Simplex Virus
• Insert Size (bp): 1131

Gene/Insert 10

• Gene/Insert name: KDM4D
• Species: Callithrix jacchus
• Insert Size (bp): 1569
• Entrez Gene: KDM4D (a.k.a. JMJD2D)

Gene/Insert 11

• Gene/Insert name: GLIS1
• Species: H. sapiens (human)
• Insert Size (bp): 1860
• Entrez Gene: GLIS1

Resource Information

• Supplemental Documents:
  • pMasterK.gb
• Addgene Notes:
  • Addgene Diagnostic Digest

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that this plasmid contains repetitive elements which are prone to recombination. Screen 3-5 small colonies to ensure the full plasmid is selected. For example, the restriction enzyme NheI can be used to check the plasmid size as shown in Addgene's Diagnostic Digest.