Myc-Xdsh-pCS2 (RXF)
(Plasmid #15490)

• Depositing Lab: Sergei Sokol
• Publication: Sokol et al Curr Biol. 1996 Nov 1. 6(11):1456-67.

Backbone

• Vector backbone: pCS2
• Backbone size w/o insert (bp): 4300
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Dishevelled
• Alt name: Xdsh
• Species: X. laevis (frog)
• Insert Size (bp): 3300
• GenBank ID: U31552
• Entrez Gene: dvl2.L (a.k.a. XELAEV_18019661mg, dishevelled, dsh, dvl, dvl2, xdd1, xdsh)
• Tag / Fusion Protein:
  • myc (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: SP6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

See author's map for details. 1 kb EcoRI-ApaI plus 2.3 kb ApaI-SalI fragments of Xdsh cDNA were cloned into 4.3 kb EcoRI-XhoI cut pCS2-myc vector in-frame.