Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


(Plasmid #155015)


Item Catalog # Description Quantity Price (USD)
Plasmid 155015 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Vector type
    Mouse Targeting
  • Selectable markers
    Split Hygro

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
  • Species
    M. musculus (mouse)
  • Mutation
    M24T (please see depositor comments)
  • Entrez Gene
    Ccn2 (a.k.a. Ctgf, Fisp12, Hcs24, fisp-12)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Mlu (not destroyed)
  • 3′ cloning site SnaBI (not destroyed)

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene NGS result had mutation M24T on Ccn2 [NP_034347.2], depositor confirmed that the mutation affects none of the key known functional domains and is not really a concern for the activity of the protein.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDragon-Ctgf was a gift from Alberto Rosello-Diez (Addgene plasmid # 155015 ; ; RRID:Addgene_155015)
  • For your References section:

    A collection of genetic mouse lines and related tools for inducible and reversible intersectional mis-expression. Ahmadzadeh E, Bayin NS, Qu X, Singh A, Madisen L, Stephen D, Zeng H, Joyner AL, Rosello-Diez A. Development. 2020 May 28;147(10). pii: dev.186650. doi: 10.1242/dev.186650. 10.1242/dev.186650 PubMed 32366677