pDragon-Ctgf
(Plasmid #155015)

• Purpose: For Flp-mediated cassette exchange. Expresses tdTomato in the presence of (r)tTA, switching to connective tissue growth factor in the presence of Cre and (r)tTA activity.
• Depositing Lab: Alberto Rosello-Diez
• Publication: Ahmadzadeh et al Development. 2020 May 28;147(10). pii: dev.186650. doi: 10.1242/dev.186650.

Backbone

• Vector backbone: AR52
• Vector type: Mouse Targeting
• Selectable markers: Split Hygro

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Ccn2
• Alt name: Ctgf
• Species: M. musculus (mouse)
• Mutation: M24T (please see depositor comments)
• Entrez Gene: Ccn2 (a.k.a. Ctgf, Fisp12, Hcs24, fisp-12)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Mlu (not destroyed)
• 3′ cloning site: SnaBI (not destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene NGS result had mutation M24T on Ccn2 [NP_034347.2], depositor confirmed that the mutation affects none of the key known functional domains and is not really a concern for the activity of the protein.