pLenti6.2_mCherry_Grx1_roGFP2
(Plasmid #155045)

• Purpose: Fluorescent redox reporter
• Depositing Lab: Vanessa LaPointe
• Publication: Plasmids expressing redox reporters coupled to fluorescent protein mCherry for lentiviral approaches (unpublished)

Backbone

• Vector backbone: pLenti6.2
• Total vector size (bp): 9794
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Glutaredoxin-1
• Alt name: GLRX1_HUMAN
• Species: H. sapiens (human)
• Entrez Gene: GLRX (a.k.a. GRX, GRX1)
• Promoter: CMV
• Tag / Fusion Protein:
  • roGFP2 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: AscI (not destroyed)
• 5′ sequencing primer: CMV-FW
• 3′ sequencing primer: WPRE-RV

Resource Information

• Supplemental Documents:
  • pLenti6.2_mCherry-GRX1-roGFP2.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry