pSEVA2BE-S
(Plasmid #155268)

• Purpose: E.coli-Pseudomonas putida KT2440 shuttle plasmid, which can be used to achieve base editing by mutating target Cytosines into Guanines in multiple Pseudomonas species.
• Depositing Lab: Jianping Wu
• Publication: CRISPR-assisted multiplex base editing system in Pseudomonas putida KT2440 (unpublished)

Backbone

• Vector backbone: pSEVA258
• Backbone size w/o insert (bp): 11000
• Vector type: Bacterial Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: APOBEC1-nCas9-UGI
• Species: S. pyogenes MGAS5005
• Insert Size (bp): 5112
• Promoter: Arabinose inducible promoter

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: Unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pSEVA6BE-S and pSEVA2BE-S are E.coli-Pseudomonas putida KT2440 shuttle plasmids, which can be used to achieve base editing by mutating target Cytosines into Guanines in multiple Pseudomonas species. pSEVA6BE-S contains pRO1600/ColE1 serving as replication protein,sgRNA targeting specific spacers, APOBEC1-nCas9D-UGI complex catalyzing Cytosines into Guanines and counter-selection marker SacB using for plasmid curing. pSEVA6BE-S contains RSF1010 serving as replication protein, sgRNA targeting specific spacers, APOBEC1-nCas9D-UGI complex catalyzing Cytosines into Guanines and counter-selection marker SacB using for plasmid curing. The base editing scope of pSEVA6BE-S and pSEVA2BE-S are mainly from C3 to C8 position in a N20 sequence with the NGG PAM.
pSEVA6BE-NG-S and pSEVA6BE-YE1-S are derivative plasmid of pSEVA6BE-S. The base editing scope of pSEVA6BE-NG-S is mainly from C3 to C8 position in a N20 sequence with the NG PAM. The base editing scope of pSEVA6BE-YE1-S is mainly from C3 to C6 position in a N20 sequence with the NGG PAM.