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pXT-PsecG-secG-secY-4xpylT-PsigxB-5'prsA-3'-LacI
(Plasmid #155300)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 155300 Standard format: Plasmid sent in bacteria as agar stab 1 $89

Backbone

  • Vector backbone
    pXT
  • Backbone manufacturer
    Isabelle Derre
  • Backbone size w/o insert (bp) 7000
  • Vector type
    Bacterial Expression ; Genomic Integration
  • Selectable markers
    Lincomycin for B. subtilis

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    secG, secY, prsA, 4xpylT
  • Species
    other
  • Insert Size (bp)
    3300
  • Promoter PsecG, PsigX

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Multiple (not destroyed)
  • 3′ cloning site Multiple (not destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pXT-PsecG-secG-secY-4xpylT-PsigxB-5'prsA-3'-LacI was a gift from Sabine Schneider (Addgene plasmid # 155300)

  • For your References section:

    Genetic Code Expansion, Protein Expression, and Protein Functionalization in Bacillus subtilis. Scheidler CM, Vrabel M, Schneider S. ACS Synth Biol. 2020 Mar 20;9(3):486-493. doi: 10.1021/acssynbio.9b00458. Epub 2020 Feb 18. 10.1021/acssynbio.9b00458 PubMed 32053368
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