pJBJZ_001
(Plasmid
#155357)
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Purpose(Empty Backbone) Gentamycin derivative of lambda-red pKD3 plasmid from Datsenko and Wanner (2000)
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 155357 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepKD3
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Backbone manufacturerWanner and Datsenko
- Backbone size (bp) 3104
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Modifications to backboneReplacement of CamR by Gentamycin resistance cassette between priming site 1 and common priming site c2. It was done by PCR amplification and HiFi assembly.
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Vector typeSynthetic Biology
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Selectable markersGentamicin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Pir1
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Growth instructionsThe plasmid can be maintained in Pir2 strains as well.
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Copy numberUnknown
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Amplification of the R cassette for lambda-red recombination can be done with or without FRT.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJBJZ_001 was a gift from Elisabeth Raleigh (Addgene plasmid # 155357 ; http://n2t.net/addgene:155357 ; RRID:Addgene_155357) -
For your References section:
Reassembling a cannon in the DNA defense arsenal: Genetics of StySA, a BREX phage exclusion system in Salmonella lab strains. Zaworski J, Dagva O, Brandt J, Baum C, Ettwiller L, Fomenkov A, Raleigh EA. PLoS Genet. 2022 Apr 4;18(4):e1009943. doi: 10.1371/journal.pgen.1009943. eCollection 2022 Apr. 10.1371/journal.pgen.1009943 PubMed 35377874
Map uploaded by the depositor.
