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GAL FLAG103QDProGFPp303
(Plasmid #15587)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 15587 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    htt 103Q Delta Pro
  • Alt name
    htt 103Q
  • Species
    H. sapiens (human)
  • Mutation
    Proline-rich region removed
  • GenBank ID
    NM_002111
  • Entrez Gene
    HTT (a.k.a. HD, IT15, LOMARS)
  • Tags / Fusion Proteins
    • FLAG (N terminal on insert)
    • GFP (C terminal on insert)

Cloning Information

Resource Information

Depositor Comments

Figure 1C in article.

Lindquist lab number: 7720

Due to slippage when polymerases go through them, the Q stretches often get shorter over time due to mistakes while they are replicated in the cells. The shortening should not have much of an effect until the Q stretches get into the 80s.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GAL FLAG103QDProGFPp303 was a gift from Susan Lindquist (Addgene plasmid # 15587 ; http://n2t.net/addgene:15587 ; RRID:Addgene_15587)
  • For your References section:

    Flanking sequences profoundly alter polyglutamine toxicity in yeast. Duennwald ML, Jagadish S, Muchowski PJ, Lindquist S. Proc Natl Acad Sci U S A. 2006 Jul 18. 103(29):11045-50. 10.1073/pnas.0604547103 PubMed 16832050