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Sup35 ScNM A230C His7
(Plasmid #15598)


Item Catalog # Description Quantity Price (USD)
Plasmid 15598 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 3200
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    Sup35 NM
  • Alt name
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
  • Mutation
    A230C. Alanine 230 mutated to cysteine.
  • Entrez Gene
    SUP35 (a.k.a. YDR172W, GST1, PNM2, SAL3, SUF12, SUP2, SUP36)
  • Tag / Fusion Protein
    • 7xHis (C terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Jonathan Weissman's lab.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Protein expression inducible by IPTG. Sc = Saccharomyces cerevisiae, NM = N-terminal and Middle domains of Sup35.

Lindquist plasmid # 8631.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Sup35 ScNM A230C His7 was a gift from Susan Lindquist (Addgene plasmid # 15598 ; ; RRID:Addgene_15598)
  • For your References section:

    Prion recognition elements govern nucleation, strain specificity and species barriers. Tessier PM, Lindquist S. Nature. 2007 May 31. 447(7144):556-61. 10.1038/nature05848 PubMed 17495929