Plas-gRNA-LEU2
(Plasmid
#157656)
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PurposeGFP and gRNA tag for protein and mRNA quantification, to attache to the 3' end of the gene coding sequence
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Depositing Lab
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Depositing OrganizationUniversity of Minnesota
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 157656 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 157656-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 157656-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepTPGI
- Backbone size w/o insert (bp) 1861
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Vector typeInsert storage (replicative in E. coli)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGFP-gRNA
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)3014
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Tag
/ Fusion Protein
- GFP-gRNA (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer NA
- 3′ sequencing primer NA
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 157656-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 157656-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Plas-gRNA-LEU2 was a gift from Frank Albert (Addgene plasmid # 157656 ; http://n2t.net/addgene:157656 ; RRID:Addgene_157656) -
For your References section:
Simultaneous quantification of mRNA and protein in single cells reveals post-transcriptional effects of genetic variation. Brion C, Lutz SM, Albert FW. Elife. 2020 Nov 16;9. pii: 60645. doi: 10.7554/eLife.60645. 10.7554/eLife.60645 PubMed 33191917
Map uploaded by the depositor.
