PurposeCRISPR reporter for mRNA quantification, integrative plasmid into NPR2 gene
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||157658||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2323
- Total vector size (bp) 11799
Vector typeInsert storage (replicative in E. coli)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert namedCAS9-VP64, mCherry, KanMX
SpeciesS. cerevisiae (budding yeast)
Insert Size (bp)11799
MutationN28D in mCherry- please see depositor comments below
- Cloning method Gibson Cloning
- 5′ sequencing primer NA
- 3′ sequencing primer NA (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Depositor confirms mutation in mCherry and single base pair deletion in Npr2p is not a concern for plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Plas-CRISPR_reporter was a gift from Frank Albert (Addgene plasmid # 157658 ; http://n2t.net/addgene:157658 ; RRID:Addgene_157658)
For your References section:Simultaneous quantification of mRNA and protein in single cells reveals post-transcriptional effects of genetic variation. Brion C, Lutz SM, Albert FW. Elife. 2020 Nov 16;9. pii: 60645. doi: 10.7554/eLife.60645. 10.7554/eLife.60645 PubMed 33191917