Plas-CRISPR_reporter
(Plasmid #157658)

• Purpose: CRISPR reporter for mRNA quantification, integrative plasmid into NPR2 gene
• Depositing Lab: Frank Albert
• Publication: Brion et al Elife. 2020 Nov 16;9. pii: 60645. doi: 10.7554/eLife.60645.

Backbone

• Vector backbone: pTPGI
• Backbone size w/o insert (bp): 2323
• Total vector size (bp): 11799
• Vector type: Insert storage (replicative in E. coli)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: dCAS9-VP64, mCherry, KanMX
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 11799
• Mutation: N28D in mCherry- please see depositor comments below

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: NA
• 3′ sequencing primer: NA

Resource Information

• A portion of this plasmid was derived from a plasmid made by: https://www.addgene.org/49013/

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Depositor confirms mutation in mCherry and single base pair deletion in Npr2p is not a concern for plasmid function.