Rictor-Myc
(Plasmid #157931)

• Purpose: Over expression of Rictor
• Depositing Lab: Baoji Xu
• Publication: Xu et al Nat Commun. 2019 Aug 9;10(1):3622. doi: 10.1038/s41467-019-11575-1.

Backbone

• Vector backbone: pUltra
• Backbone size w/o insert (bp): 8326
• Total vector size (bp): 12700
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Rictor
• Species: M. musculus (mouse)
• Insert Size (bp): 5127
• GenBank ID: NM_030168.3
• Entrez Gene: Rictor (a.k.a. 4921505C17Rik, 6030405M08Rik, AVO3, D530039E11Rik)
• Promoter: Ubiquitinc Promoter
• Tags / Fusion Proteins:
  • Myc (C terminal on insert)
  • His (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (not destroyed)
• 3′ cloning site: NheI (not destroyed)
• 5′ sequencing primer: hUBCpro-F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry