PurposeVisualization of actin in mammalian cells using mGold
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||158009||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Total vector size (bp) 5778
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneACTB (a.k.a. BRWS1, PS1TP5BP1)
- Promoter CMV
/ Fusion Protein
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Existing fluorescent protein in plasmid Adggene# 56395 was replaced with mGold.
"C-18" of the name of the plasmid indicates the position of the tag (N- or C-terminal with respect to the fluorescent protein) and the number of residues in the linker between the two proteins. Please note that the mVenus annotation in the plasmid map is incorrect, as this plasmid encodes mGold.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCMV-mGold-Actin-C-18 was a gift from Francois St-Pierre (Addgene plasmid # 158009 ; http://n2t.net/addgene:158009 ; RRID:Addgene_158009)
For your References section:Versatile phenotype-activated cell sorting. Lee J, Liu Z, Suzuki PH, Ahrens JF, Lai S, Lu X, Guan S, St-Pierre F. Sci Adv. 2020 Oct 23;6(43). pii: 6/43/eabb7438. doi: 10.1126/sciadv.abb7438. Print 2020 Oct. 10.1126/sciadv.abb7438 PubMed 33097540