Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


pSI-356 v2
(Plasmid #158431)


Item Catalog # Description Quantity Price (USD)
Plasmid 158431 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size (bp) 2627
  • Vector type
    Mammalian Expression, CRISPR
  • Promoter Human H6

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    lentiGuide-Puro (

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For the cloning of gRNA, two BsmbI sites flanked by 2kb filler sequences can be used for Golden Gate Assembly.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSI-356 v2 was a gift from Nozomu Yachie (Addgene plasmid # 158431 ; ; RRID:Addgene_158431)
  • For your References section:

    Highly Multiplexed Analysis of CRISPR Genome Editing Outcomes in Mammalian Cells. Ishiguro S, Yachie N. Methods Mol Biol. 2021;2312:193-223. doi: 10.1007/978-1-0716-1441-9_12. 10.1007/978-1-0716-1441-9_12 PubMed 34228292