Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.
We have implemented updates to our Transparency and Privacy Policy.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

(Plasmid #158738)


Item Catalog # Description Quantity Price (USD)
Plasmid 158738 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 3802
  • Total vector size (bp) 7705
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy


  • Gene/Insert name
  • Species
  • Insert Size (bp)
  • Mutation
    Truncated form of TEV protease (220-242 amino acids deleted, low affinity). S219V mutation improves stability, S153N mutation improves catalytic activity. Calmodulin from GCaMP5
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag, V5

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    scFLARE1 was a gift from Alice Ting (Addgene plasmid # 158738 ; ; RRID:Addgene_158738)
  • For your References section:

    Transcriptional readout of neuronal activity via an engineered Ca(2+)-activated protease. Sanchez MI, Nguyen QA, Wang W, Soltesz I, Ting AY. Proc Natl Acad Sci U S A. 2020 Dec 15. pii: 2006521117. doi: 10.1073/pnas.2006521117. 10.1073/pnas.2006521117 PubMed 33323488