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scFLARE1
(Plasmid #158738)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 158738 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAAV
  • Backbone size w/o insert (bp) 3802
  • Total vector size (bp) 7705
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    scFLARE1
  • Species
    Synthetic
  • Insert Size (bp)
    3900
  • Mutation
    Truncated form of TEV protease (220-242 amino acids deleted, low affinity). S219V mutation improves stability, S153N mutation improves catalytic activity. Calmodulin from GCaMP5
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag, V5

Cloning Information

Resource Information

  • Supplemental Documents
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    scFLARE1 was a gift from Alice Ting (Addgene plasmid # 158738 ; http://n2t.net/addgene:158738 ; RRID:Addgene_158738)
  • For your References section:

    Transcriptional readout of neuronal activity via an engineered Ca(2+)-activated protease. Sanchez MI, Nguyen QA, Wang W, Soltesz I, Ting AY. Proc Natl Acad Sci U S A. 2020 Dec 15. pii: 2006521117. doi: 10.1073/pnas.2006521117. 10.1073/pnas.2006521117 PubMed 33323488