EGFP-UTRN-mini
(Plasmid
#158749)
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PurposeExpresses a shorter version of Utrophin (amino acid 35-136) with amino terminus eGFP in mammalian cells
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Depositing Lab
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Depositing OrganizationInstitute for Stem Cell Science and Regenerative Medicine (inStem), India
Located in India -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 158749 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 158749-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 158749-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCS2+
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Backbone manufacturerBurkel et al Cell Motil Cytoskeleton. 2007 Nov . 64(11):822-32.
- Backbone size w/o insert (bp) 4823
- Total vector size (bp) 5123
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Modifications to backboneInserted UTRN-mini gene after eGFP in the Addgene plasmid # 26737 by Gibson cloning.
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameUTRN-mini
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Alt nameUtrophin-mini
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Alt nameShorter actin binding Utrophin variant (amino acid 35-136)
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Insert Size (bp)309
- Promoter CMV IE94
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Tag
/ Fusion Protein
- eGFP (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer SP6
- 3′ sequencing primer EBV-rev
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byReceived the plasmid # 26737 from Addgene and introduced UTRN-mini gene after eGFP by Gibson cloning.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 158749-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 158749-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
EGFP-UTRN-mini was a gift from Minhajuddin Sirajuddin (Addgene plasmid # 158749 ; http://n2t.net/addgene:158749 ; RRID:Addgene_158749) -
For your References section:
Structural insights into actin filament recognition by commonly used cellular actin markers. Kumari A, Kesarwani S, Javoor MG, Vinothkumar KR, Sirajuddin M. EMBO J. 2020 Jun 22:e104006. doi: 10.15252/embj.2019104006. 10.15252/embj.2019104006 PubMed 32567727
Map uploaded by the depositor.
