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EGFP-UTRN-mini
(Plasmid #158749)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 158749 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCS2+
  • Backbone manufacturer
    Burkel et al Cell Motil Cytoskeleton. 2007 Nov . 64(11):822-32.
  • Backbone size w/o insert (bp) 4823
  • Total vector size (bp) 5123
  • Modifications to backbone
    Inserted UTRN-mini gene after eGFP in the Addgene plasmid # 26737 by Gibson cloning.
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    UTRN-mini
  • Alt name
    Utrophin-mini
  • Alt name
    Shorter actin binding Utrophin variant (amino acid 35-136)
  • Insert Size (bp)
    309
  • Promoter CMV IE94
  • Tag / Fusion Protein
    • eGFP (N terminal on insert)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Received the plasmid # 26737 from Addgene and introduced UTRN-mini gene after eGFP by Gibson cloning.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EGFP-UTRN-mini was a gift from Minhajuddin Sirajuddin (Addgene plasmid # 158749 ; http://n2t.net/addgene:158749 ; RRID:Addgene_158749)
  • For your References section:

    Structural insights into actin filament recognition by commonly used cellular actin markers. Kumari A, Kesarwani S, Javoor MG, Vinothkumar KR, Sirajuddin M. EMBO J. 2020 Jun 22:e104006. doi: 10.15252/embj.2019104006. 10.15252/embj.2019104006 PubMed 32567727