PurposeT7 expression of C-term fluorescent fusion in E. coli
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||158999||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typeBacterial Expression ; Integrating Mycobacterial Expression Vector attL5
Growth in Bacteria
Bacterial Resistance(s)Apramycin, 25 μg/mL
Copy numberHigh Copy
- Promoter pmycTetO
/ Fusion Protein
- Gly-Ala Linker/Dendra2/1x Flag Tag (C terminal on insert)
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer TGATAGAGTTTGTCCTCCCTATCAG
- 3′ sequencing primer TCCATATGCACCTTGACACG (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
NCBI reference sequence: CP000480.1. 5' cloning site: Ase1, destroyed during cloning. 3' cloning site: HindIII, not destroyed during cloning
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMSR:MSMEG_0034:Dendra was a gift from Keith Derbyshire & Joseph Wade (Addgene plasmid # 158999 ; http://n2t.net/addgene:158999 ; RRID:Addgene_158999)
For your References section:A Mycobacterial Systems Resource for the Research Community. Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A, Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade JT, Derbyshire KM. mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 10.1128/mBio.02401-20. 10.1128/mBio.02401-20 PubMed 33653882