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(Plasmid #159108)


Item Catalog # Description Quantity Price (USD)
Plasmid 159108 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Vector type

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number


  • Gene/Insert name
  • Species
    H. sapiens (human)
  • Promoter UBC

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CCAGGTGCCATTGATGGTGTCATT
  • 3′ sequencing primer TAGAAGATGTGTTGGGCAGCAGTG
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    P2Y2R complementary DNA was a gift of Dr. Marta Fumagalli, University of Milan, Department of Pharmacological and Biomolecular Sciences, Laboratory of Molecular and Cellular Pharmacology of Purinergic Transmission. Address: Via Balzaretti, 9/11/13 - 20133 MILANO (MI), Italy.
  • Article Citing this Plasmid

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHOT-P2Y2R was a gift from Fabio Mammano (Addgene plasmid # 159108 ; ; RRID:Addgene_159108)
  • For your References section:

    Organ-on-chip model shows that ATP release through connexin hemichannels drives spontaneous Ca(2+) signaling in non-sensory cells of the greater epithelial ridge in the developing cochlea. Mazzarda F, D'Elia A, Massari R, De Ninno A, Bertani FR, Businaro L, Ziraldo G, Zorzi V, Nardin C, Peres C, Chiani F, Tettey-Matey A, Raspa M, Scavizzi F, Soluri A, Salvatore AM, Yang J, Mammano F. Lab Chip. 2020 Jul 23. doi: 10.1039/d0lc00427h. 10.1039/d0lc00427h PubMed 32700707